"Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection" just got published in Microbial Biotechnology.
The article is open access!
The present protocol describes a streamlined method for precise genome engineering of Pseudomonas species. By using a combination of fluorescent markers, coupled with the activity of the I‐SceI meganuclease or CRISPR/Cas9‐mediated counterselection, virtually any genomic manipulation can be performed in 3–5 days.