Brief Report Published in Microbial Biotechnology

“Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida” just got published in Microbial Biotechnology. The article is open access!   This study describes the re‐purposing of the elements within the classical XylS/Pm system into a novel gene expression device which enables extremely tight expression control. The combination of the system…

Special Issue Article in Microbial Biotechnology

“Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection” just got published in Microbial Biotechnology. The article is open access!   The present protocol describes a streamlined method for precise genome engineering of Pseudomonas species. By using a combination of fluorescent markers, coupled with the activity of the I‐SceI meganuclease or CRISPR/Cas9‐mediated counterselection, virtually any genomic manipulation…