Construction of P. putida cell factories for bioproduction of fluoropolymers
WP4 will set the basis for in vivo fluoropolymer production in P. putida by engineering PFHA synthesis in bacteria fed with fluorinated precursors and cheap carboxylates, and these activities will proceed in parallel to WP3. Metabolic manipulations will be implemented to ensure high polymer accumulation. Since different fluoropolymers will be tested at this stage, the physicochemical properties of the materials will be analyzed to focus efforts on the most promising types of PFHA.