Publications

MP Mezzina, MT Manoli, MA Prieto, PI Nikel. 2020. Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates. Biotechnology Journal,
https://doi.org/10.1002/biot.202000165

Markakis K, Lowe PT, Davison-Gates L, O’Hagan D, Rosser SJ, Elfick A. 2020. An Engineered E. coli Strain for Direct in Vivo Fluorination. ChemBioChem, https://doi.org/10.1002/cbic.202000051

Calero P, Volke DC, Lowe PT et al. 2020. A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida. Nature Communications, https://doi.org/10.1038/s41467-020-18813-x

Nieto-Domínguez M, Nikel PI. 2020. Intersecting Xenobiology and Neometabolism To Bring Novel Chemistries to Life. ChemBioChem, https://doi.org/10.1002/cbic.202000091

Babkova P, Dunajova Z, Chaloupkova R, Damborsky J, Bednar D, Marek M. 2020. Structures of hyperstable ancestral haloalkane dehalogenases show restricted conformational dynamics. Computational and Structural Biotechnology Journal, https://doi.org/10.1016/j.csbj.2020.06.021

Batianis C, Kozaeva E, Damalas SG, Martín‐Pascual M, Volke DC, Nikel PI, Martins dos Santos VAP. 2020. An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida. Microbial Biotechnology, https://doi.org/10.1111/1751-7915.13533

Volke DC, Friis L, Wirth NT, Turlin J, Nikel PI. 2020. Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida. Metabolic Engineering Communications, https://doi.org/10.1016/j.mec.2020.e00126.
Demko M, Chrást L, Dvořák P, Damborský J, Šafránek D. 2019. Computational Modelling of Metabolic Burden and Substrate Toxicity in Escherichia coli Carrying a Synthetic Metabolic Pathway. Microorganisms. https://doi.org/10.3390/microorganisms7110553

Schmidt M, Budisa N. 2020. Alternative Biofacts – Life as we don’t (yet) know it.
Chapter in the book “Art as We Don’t Know It”. PDF

Arce‐Rodríguez A, Volke DC,  Bense S,  Häussler S,  Nike PI. 2019. Non‐invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator. Microbial Biotechnology Vol.12(4): 799-813. https://doi.org/10.1111/1751-7915.13439

 Evolutionary Approaches for Engineering Industrially Relevant Phenotypes in Bacterial Cell Factories. Biotechnology Journal. https://doi.org/10.1002/biot.201800439

Wirth NT, Kozaeva E, Nikel PI, 2019. Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection.  Microbial Biotechnology. https://doi.org/10.1111/1751-7915.13396

Martinelli L, Nikel PI. 2019. Breaking the state‐of‐the‐art in the chemical industry with new‐to‐Nature products via synthetic microbiology. Microbial Biotechnology. Vol. 12(2): 187-190. https://doi.org/10.1111/1751-7915.13372